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Dupont Industrial Biosciences / Genencor International BVBA

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Abstract Bachelor Project 1 FBT 2018-2019Rapid, automatic and innovative micro biology after validation of end products by means of Soleris vials in a BioLumix machine

Enzymes act as catalysts in many biochemical processes. In Genencor International - DuPont Bruges, three kinds of microorganisms are used to produce enzymes: bacteria, yeast and molds. It is extremely important that the final product, the enzymes, are completely uncontaminated  by these microorganisms. This can be tested in two ways: by carrying out a manual analysis on so-called ‘plates’ or by exercising an analysis by means of vials in a BioLumix machine. Until recently the latter analysis was performed by using BioLumix vials in Genencor International - DuPont plants. As these vials are no longer produced, Genencor International – DuPont started testing Soleris vials in order to decide whether these can be a trustworthy alternative in the future.

The aim of the present investigation, consequently, is to determine the absolute accuracy of the analyses of yeast, molds and TVC (total viable count) made by means of Soleris vials. This will be achieved by comparing these analyses with the results of the traditional manual analyses on plates. If these validation tests are positive, this will allow Genencor International - DuPont to make the necessary tests on the produced enzymes by means of the BioLumix machine again, with Soleris vials, which is performed a lot quicker and hence a lot cheaper than the manual analyses.The validation tests will be done on three samples of the same end product containing enzymes both in the BioLumix machine and on plates. The number of detected microorganisms by means of Soleris vials in the BioLumix machine should agree with the numerical values observed on the plates.

The results of the BioLumix tests indicate whether the dilution is larger or smaller than the dilution applied in the vial. The results of the manual analyses on the plate are expressed in numerical values, indicating the size of the recorded growth of microorganisms. If the values recorded by the BioLumix machine tally with those of the manual analyses, then the validation may be called successful. The results of this investigation and those in several other plants of Genencor International - DuPont have been collected and compared with each other in the United States. Already in the month of May of this year the conclusion was reached that the attempts to re-validate products on the BioLumix machine using the Soleris vials had been hardly successful and did not lead to great confidence in the Soleris vial type.

Consequently, Genencor International - DuPont has decided to suspend the validations for TAC, yeast and mold Soleris vials in the BioLumix machine immediately in all plants. The use of Soleris vials is considered to cause greater challenges than benefits presently. Genencor International - DuPont is now looking at The Global Microbiology Group for rapid microbiology methods as a solution of the problem.

 

Abstract Bachelor Project 2 FBT 2018-2019Validation infrared technique using an auto analyzer

The analytical lab of Genencor International determines the enzyme activity of fermentation samples. The enzyme activity can be determined with a near infrared equipment and an autoanalyzer. A validation of a near infrared equipment was done during the internship period. This validation needs to happen every three years because the composition of the product can be changed. In addition, the validation ensures a more qualitative and stabile determination.

The validation checks if the calibration curve is still up to date for the samples which have been produced. At least three samples need to be measured for each fermentation with the near infrared device. The measured values are a prediction of the enzyme activity. The reference activity is determined from the same samples with an autoanalyzer. The validation gets approved as soon as the difference of both values is less than 5 %.

The near infrared device was validated for six different fermentations. The difference between the reference values and the predicted values is more than 5 % for five fermentations. This means that the five calibration curves should be updated. The other fermentation has been approved.

 

Abstract bachelor project FBT 2017-2018: Efficient microbiology using the BioLumix. Validation of enzyme-containing end products

Enzymes are biological catalysts that are significant in all essential biochemical processes. Because of their specific advantages, enzymes are increasingly used in various applications in the pursuit of a sustainable society. Due to the increasing demand, the production of enzymes is industrialized. In Genencor International-Dupont enzymes are produced from microorganisms.  The enzyme-containing end products must be free from contamination, impurities and the producing microorganism.  Therefore microbial testing is needed throughout the whole production process. The classic plate method is labor intensive, time consuming and the subjective interpretation of results can also be a disadvantage. The use of the BioLumix, a rapid microbiological method, can offer a solution because this method provides quicker and objective results.

 

Abstract bachelorproef 2016-2017Introduction for the determination of the enzyme activity with the micro viscometer Lovis

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Abstract bachelorproef 2015-2016Kalibratie en validatie IR techniek met behulp van een auto-analyser

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Address

Komvest 43
8000 Brugge
050449204
Belgium
Komvest 43
8000 Brugge
050449204
Belgium

Contacts

Traineeship supervisor
Goedele Seynaeve
goedele.seynaeve@dupont.com
Traineeship supervisor
Ilse Breusegem
Traineeship supervisor
Tine Devos
Chris Vermeersch
Traineeship supervisor
Ilse Swennen
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