Abstract bachelorproef 2016-2017: Direct detection of testosterone esters in sports, Development of an ultra-sensitive GC/MSMS method for the detection of testosterone esters in blood plasma
The goal of this study was to develop an ultra-sensitive method for detecting T esters in blood plasma. The reason for the development is, that the detection of an intact ester of testosterone could lead towards unequivocal proof of misuse through administration of the exogenous testosterone.
In this study, an ultra-sensitive detection method was created on GC/MSMS including a custom sample preparation protocol. In our study protocol a liquid-liquid extraction (LLE) was used for the sample clean-up. Furthermore the use of a HPLC with a fraction collector was used to exclude the samples of cholesterol.
After the method development, it was validated according to the in-house validation protocol of DoCoLab.
The method was then tested on blood samples of 12 volunteers. These were collected in a Nebido® study. In this study volunteers were injected with a single dose of 1g of Nebido®, a drug containing T undecanoate. After the administration, blood samples were collected over a time period of 3 months.
Through this method it was possible to detect T undecanoate between 32 and 86 days. This is a similar result as the available methods in up-to-date literature. In the future the interference of cholesterol could be eliminated which would result in clean and better compound response. Still the method can detect 8 other T esters, so it could be used as direct method which in the future could result in a standalone detection method or as a confirmation test. As a confirmation test it could replace the more expensive analysis with GC/C/IRMS, which would lower the cost and expensive for the analyst and the client.
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