Medisch Labo Bruyland
The aim of this study is to compare two devices (EuroBlotMaster and Phadia 250) for analysing antinuclear antibodies.
Antinuclear antibodies are autoantibodies that are used in the diagnosis of systemic autoimmune diseases such as Lupus Erythematosus, Sjögren’s syndrome, Systemic sclerosis, Mixed connective tissue disease, Polymyositis and Dermatomyositis. Examples of autoantibodies are anti-dsDNA, ant-Ro, anti-La, anti-Sm, anti-RNP, anti-Scl etc. This autoantibodies are also called antinuclear antibodies.
The first step is a screening with Hep-2000® cells. The result is a fluorescent pattern and a titer. If the titer is at least 1/80, additional tests are done. This can be done on the EuroBlotMaster or/an Phadia 250.
The Phadia 250 uses the Fluorescence Enzyme immunoassay (FEIA) technique. First there is a screening with the Symphony. If this is positive, all the autoantibodies are separately determined. Autoantibodies that are tested; anti-dsDNA, anti-CENP, anti-Jo1, anti-Scl-70, anti-Sm, anti-RNP, anti-Ro52/60, anti-La and anti-RNP-70.
The EuroBlotMaster with the immunoblot technique, uses strips which are stained by indirect immunostaining. From this, a band pattern is observed by using the EuroLineScan. This scan converts an intensity to a qualitative observation. This strip can detect sixteen autoantibodies.
There is a good agreement between the two methods for most of the tested antibodies. Another advantage of the EuroBlotMaster is the detection of six other antibodies, that can help in the further diagnostic of auto-immune diseases. The workflow for ENA detection will be changed. After a screening with Symphony on Phadia 250, positive results are further analysed with the immunoblot technique with the EuroBlotMaster.
Generally it can be decided that the results on the EuroBlotMaster and the results on the Phadia 250 are comparable.
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