Ugent faculteit diergeneeskunde, Vakgroep Pathologie, Bacteriologie en Pluimvee
Abstract bachelor project 2018-19: BATRACHOCHYTRIUM DENDROBATIDIS BIJ DE ALPENWATERSALAMANDER
Batrachochytrium dendrobatidis is a major pathogen of frogs worldwide, associated with declines in amphibian populations. The skin disease caused by this fungus is named chytridiomycosis and affects the vital function of amphibian skin. Not all amphibians respond equally to infection and host responses might range from resistant, over tolerant to susceptible. The clinical outcome of infection is highly dependent on the amphibian host, the fungal virulence and environmental determinants. B. dendrobatidis infects the skin of a large range of anurans, urodeles and caecilians. The aim of this study is to identify the impact of B. dendrobatidis infections in wild alpine newts . Data for this study were obtained from alpine newts that were trapped in fikes. This screening was done at different time points and consisted of a sampling, DNA extraction, preparation for the final qPCR and the qPCR. The results show that there’s only 1 % of the samples positive for B. dendrobatidis. This reveals that the prevalence of the fungus is very low in East Flanders. For the future, there must be keeping an eye on the B. dendrobatidis for further dispersion.
Abstract bachelor project 2016-17: Optimization of PCR with specific primers for SNP detection in fire salamanders
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Samenvatting eindwerk 2014-2015: Screening on lethal chytridiomycosis through quantitative PCR on wild living amphibians
Chytridiomycosis is an infection caused by two different fungi, Batrachochytrium dendrobatidis (Bd) or Batrachochytrium salamandrivorans (Bs). Both fungal species infect amphibians. Bs is more isolated and characterized from salamanders and Bd from frogs / toads.
About ten years ago Bd was isolated for the first time from frog skin and described. This fungus causes epidermal hyperplasia and hyperkeratosis of the amphibian skin. In 2012, another related species was discovered, the salamander-infecting fungus Bs. This fungus causes especially deep skin ulcers.
Both fungi interfere with the physiological functioning of the amphibian skin, whereby they eventually cause in most cases, death of the amphibian. The fast and easy spread of these fungi lately induces a large number of infections. There is also observing a huge decline and even extinction of some amphibian populations. Therefore chytridiomycose is one of the greatest threats to biodiversity.
Nowadays, it is important to screen as many as possible amphibians both living and dead, of the presence of these fungi. Thanks to this kind of screening more information can be discovered about the lethal chytridiomycosis. Such screenings are done through quantitative PCR. At this point, it can’t be avoided that these fungi cause extinction of some wild amphibian populations.
This screening consisted of a sampling, DNA extraction, preparation for the final qPCR and the qPCR. The different screenings were performed according to different sampling methods and DNA extractions.
First, 28 wild yellow-bellied toads were found during different periods screened on the Bd fungus. This has shown that there was only one toad positive. However, it was in fact a small dose, and it was not certain whether the toad was recently infected or whether it was already in a recovery phase. The toad wasn’t found later, there is no certainty that this animal has survived the infection. The next screenings were performed on amphibians that were found in the wild death. These screenings are especially important to quickly detect an infection outbreak. Both dead newt and several ordinary toads were screened. None of these animals had chytridiomycose as cause of death.
These screenings could clarify why and how a chytridiomycosis outbreak takes place specifically on these locations. Also changes in infection status from some animals can be checked. Because of this, a solution to this problem could eventually be found on a long-term perspective.
Prof. Ann Martel