AZ Damiaan Oostende
Legionella pneumophila is a bacteria that can cause serious diseases in humans such as the Veteran disease and the Pontiac fever. It is therefore important to diagnose this bacteria quickly before irreversible damage occurs to the patient. It is not possible yet to determine the bacteria through urine antigen-detection in the AZ Damiaan lab. The aim of this study is therefore to compare three kits for the determination of Legionella pneumophila in urine. The kit that scores best will then be routinely used by the lab.
The tested kits were BinaxNow (Abbott), ImmuView (International Medical) and CerTest (Selinion). The three kits were tested for specificity, sensitivity, PPV, NPV and accuracy. This was done using 20 positive samples. The kits were also compared based on the price-quality ratio, inclusion of the controls, ease of use, logistics and distribution.
The BinaxNow kit and the ImmuView kit both achieved high sensitivity and high specificity, while the Certest kit achieved a high sensitivity, but a low specificity. The Certest kit is the cheapest, but if the other criteria are compared, the BinaxNow kit comes out best. The kit uses a device to read samples, is easy to use, includes a positive and negative control as standard and the company already supplies other kits to the lab.
The Abbott BinaxNow kit scored best on both statisticall and on other criteria tested. This kit will therefore be included in the routine of the lab
AZ Damiaan Oostende wants to perform the detection of Varicella zoster virus antibodies in-house on the DS2 platform from Dynex®. The aim of this validation is to evaluate two ELISA kits from different companies (EuroImmun and Vircell).
The detection of Varicella zoster virus antibodies is important for several patients. Pregnant women who get in contact with the virus, need to know their immune status for the protection of their unborn child. There are several kits available for detection.
Different samples were validated and compared on both kits in a short time-frame. Several runs were performed on the DS2 platform with the same samples under the same conditions to compare the results of both kits. The samples were tested for both IgM- and IgG-positivity and compared to the results obtained by the laboratory AML, where these tests were done on a chemiluminescence immunoassay platform. The controls of each run were tracked and compared to each other by calculating mean, standard deviation and coefficient of variation. Specific samples were tested to see if there was any cross-reactivity to Epstein-Barr virus, Herpes simplex virus, Reumafactor and Cytomegalovirus in the detection of IgM-antibodies.
The coefficients of variation were for both IgM and IgG from both kits less than 10%. There was only one sample out of 28 that was discordant for.
The IgM kit from Vircell had significant more cross-reactivity than EuroImmun.
EuroImmun IgM, IgG and Vircell IgM and IgG showed a sensitivity of 100%. The specificity was 100% for EuroImmun IgM, Vircell IgM and IgG. The kit EuroImmun IgG had a specificity of 83,3%.
Due to the lower cross-reactivity and practical considerations, the laboratory chooses to use the IgM/IgG-kits from EuroImmun.
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Apr. L. Vynckier